Description
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This S17AH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat S17AH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
PRINCIPLE OF THE ASSAY S17AH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-S17AH antibody and an S17AH-HRP conjugate. The assay sample and buffer are incubated together with S17AH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the S17AH concentration since S17AH from samples and S17AH-HRP conjugate compete for the anti-S17AH antibody binding site. Since the number of sites is limited, as more sites are occupied by S17AH from the sample, fewer sites are left to bind S17AH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S17AH concentration in each sample is interpolated from this standard curve